山羊疱疹病毒Ⅰ型的分离鉴定与致病性

doi:10.11843/j.issn.0366-6964.2018.08.026

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (8): 1797-1802.doi: 10.11843/j.issn.0366-6964.2018.08.026

• 研究简报 • 上一篇

山羊疱疹病毒Ⅰ型的分离鉴定与致病性

郝飞¹, 毛立¹, 李文良^1*, 李基棕¹, 杨蕾蕾¹, 张纹纹¹, 孙敏¹, 钟纯燕^1,2, 刘茂军¹, 江杰元^1*

1. 江苏省农业科学院兽医研究所, 农业部兽用生物制品工程重点实验室, 南京 210014;
2. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2018-01-17 出版日期:2018-08-23 发布日期:2018-08-23
通讯作者: 李文良,副研究员,博士,E-mail:kfliwenliang@163.com;江杰元,研究员,博士,E-mail:1776556843@qq.com
作者简介:郝飞(1988-),男,硕士,助理研究员,主要从事动物疫病诊断防控技术研究,E-mail:haofeijaas@163.com
基金资助:
“十三五”国家重点研发计划（2016YFD0500900）；江苏省农业科技自主创新资金项目（CX（14）2090）

Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain

HAO Fei¹, MAO Li¹, LI Wen-liang^1*, LI Ji-zong¹, YANG Lei-lei¹, ZHANG Wen-wen¹, SUN Min¹, ZHONG Chun-yan^1,2, LIU Mao-jun¹, JIANG Jie-yuan^1*

1. Key Laboratory for Veterinary Bio-Product Engineering of Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2018-01-17 Online:2018-08-23 Published:2018-08-23

摘要/Abstract

摘要：

为了查明引起2013-2014年江苏多地育肥羊出现呼吸道症状为主的疾病的病因，通过采集发病羊场鼻拭子，采用细胞接毒分离培养、病毒蚀斑纯化、病毒形态结构观察和病毒核酸鉴定，分离到一株山羊疱疹病毒Ⅰ型（caprine herpesvirus 1，CpHV-1），命名为JSHA1405；并对其进行了致病性试验。分离鉴定结果表明：病料接种MDBK细胞后，盲传至第3代时产生明显细胞病变；电镜下可见直径约100 nm有囊膜的病毒粒子；gB全基因测序表明其为CpHV-1，序列比对显示JSHA1405株与CpHV-1瑞士分离毒E/CH株相似性最高，为99.2%。致病性试验表明，JSHA1405分离株可导致山羊发热持续一周以上，体温最高可达41.8℃。山羊感染后临床症状明显，表现为精神沉郁、流浆液性或脓性鼻涕，可通过鼻腔/粪便排毒。山羊感染后第7天出现中和抗体，第28天达到最高。这是国内首次报道CpHV-1的分离鉴定及致病性，分离毒JSHA1405具有较强的致病性，为CpHV-1病原学及防控技术研究提供依据与参考。

Abstract:

This study aimed to find the cause of severe respiratory disease in fattened goat herds of many farms in Jiangsu province during 2013 to 2014. In this study, one caprine herpesvirus 1 named JSHA1405 was detected and isolated from nasal swab samples of goat herds with severe respiratory disease, and identified by transmission electron microscopy, PCR and virus pathogenicity test. MDBK cells infected with the third generation of JSHA1405 showed typical cytopathogenic effect (CPE). Under transmission electron microscopy detection, the enveloped virion could be observed with the diameter of 100 nm. Sequence analysis of gB gene indicated that JSHA1405 had some variation compared with E/CH strain, and the homology was 99.2%. Furthermore, the pathogenicity test indicated that JSHA1405 could induce high fever (peaked at 41.8℃) and lasted for about one week, other symptoms included depression, nasal discharge. Virus shedding could be detected from nasal and rectal swabs. Neutralizing antibodies appeared from the 7^th day post infection (dpi) and peaked at 28^th dpi. This is the first report about isolation, identification and pathogenicity study of CpHV-1 in China. The experiments demonstrated that the CpHV-1 strain JSHA1405 was pathogenic to goats. It provides foundation for further study of the viral etiology and the prevention and control of CpHV-1.

中图分类号:

S852.659.1

郝飞, 毛立, 李文良, 李基棕, 杨蕾蕾, 张纹纹, 孙敏, 钟纯燕, 刘茂军, 江杰元. 山羊疱疹病毒Ⅰ型的分离鉴定与致病性[J]. 畜牧兽医学报, 2018, 49(8): 1797-1802.

HAO Fei, MAO Li, LI Wen-liang, LI Ji-zong, YANG Lei-lei, ZHANG Wen-wen, SUN Min, ZHONG Chun-yan, LIU Mao-jun, JIANG Jie-yuan. Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1797-1802.

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参考文献

[1] THIRY J, KEUSER V, MUYLKENS B, et al. Ruminant alphaherpesviruses related to bovine herpesvirus 1[J]. Vet Res, 2006, 37(2):169-190.
[2] DAVISON A J. Herpesvirus systematics[J]. Vet Microbiol, 2010, 143(1):52-69.
[3] BENAVIDES J, GONZÁLEZ L, DAGLEISH M, et al. Diagnostic pathology in microbial diseases of sheep or goats[J]. Vet Microbiol, 2015, 181(1-2):15-26.
[4] ROPERTO F, PRATELLI A, GUARINO G, et al. Natural caprine herpesvirus 1(CpHV-1) infection in kids[J]. J Comp Pathol, 2000, 122(4):298-302.
[5] SAITO J K, GRIBBLE D H, BERRIOS P E, et al. A new herpesvirus isolate from goats:Preliminary report[J]. Am J Vet Res, 1974, 35:847-848.
[6] METTLER F, ENGELS M, WILD P, et al. Herpesvirus infection in kids in Switzerland[J]. Schweiz Arch Tierheilkd, 1979, 121(12):655-662.
[7] PIPER K L, FITZGERALD C J, FICORILLI N, et al. Isolation of caprine herpesvirus 1 from a major outbreak of infectious pustular vulvovaginitis in goats[J]. Aust Vet J, 2008, 86(4):136-138.
[8] SILVA M L C R, PITUCO E M, NOGUEIRA A H C, et al. Serological evidence and risk factors associated with Caprine herpesvirus 1 in dairy goat flocks in a semiarid region of northeastern Brazil[J]. J Vet Diagn Invest, 2013, 25(1):125-128.
[9] SUAVET F, CHAMPION J L, BARTOLINI L, et al. First description of infection of caprine herpesvirus 1(CpHV-1) in goats in mainland france[J]. Pathogens, 2016, 5(1):17.
[10] 李文良, 毛立, 程素平, 等. 山羊源副流感病毒3型的分离与分子鉴定[J]. 畜牧兽医学报, 2015, 46(2):344-348.
LI W L, MAO L, CHENG S P, et al. Isolation and identification of caprine derived parainfluenza virus type 3[J]. Acta Veterinaria et Zootechnica Sinica, 2015, 46(2):344-348. (in Chinese)
[11] 郝飞, 汤德元, 李春燕, 等. 伪狂犬病病毒Guizhou-DY株的分离鉴定及致病性研究[J]. 畜牧兽医学报, 2013, 44(11):1851-1856.
HAO F, TANG D Y, LI C Y, et al. Identification and pathogenicity study of pseudorabies virus Guizhou-DY strain[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(11):1851-1856. (in Chinese)
[12] REED L J, MUENCH H. A simple method of estimating fifty percent endpoints[J]. Am J Epidemiol, 1938, 27(3):493-497.
[13] CHAKRABORTY S, KUMAR A, TIWARI R, et al. Advances in diagnosis of respiratory diseases of small ruminants[J]. Vet Med Int, 2014, 2014:508304.
[14] BANYARD A, WANG Z L, PARIDA S. Peste des petits ruminants virus, eastern Asia[J]. Emerg Infect Dis, 2014, 20(12):2176-2178.
[15] 许健, 储岳峰, 高鹏程, 等. 我国17株绵羊肺炎支原体分子分型及其菌体蛋白免疫印迹[J]. 微生物学报, 2011, 51(10):1421-1426.
XU J, CHU Y F, GAO P C, et al. Molecular typing and immunoblotting of 17 Mycoplasma ovipneumoniae isolates from China[J]. Acta Microbiologica Sinica, 2011, 51(10):1421-1426. (in Chinese)
[16] 赵萍, 陈胜利, 郝华芳, 等. 一种山羊支原体山羊肺炎亚种多重抗原肽的小鼠免疫试验[J]. 畜牧兽医学报, 2016, 47(12):2476-2482.
ZHAO P, CHEN S L, HAO H F, et al. Immune responses in mice immunized with a recombinant multiple antigenic peptide from Mycoplasma capricolum Subsp. capripneumoniae[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(12):2476-2482. (in Chinese)
[17] 徐春光, 郝永清, 郝瑞霞, 等. 丝状支原体山羊亚种PG3株的全基因组序列测定与分析[J]. 微生物学通报, 2017, 44(11):2669-2678.
XU C G, HAO Y Q, HAO R X, et al. Complete genome sequence and analysis of Mycoplasma mycoides subsp. capri str. PG3[J]. Microbiology China, 2017, 44(11):2669-2678. (in Chinese)
[18] DASSANAYAKE R P, SHANTHALINGAM S, SUBRAMANIAM R, et al. Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia[J]. Vet Microbiol, 2013, 162(1):166-172.
[19] BERTOLINI S, ROSAMILIA A, CARUSO C, et al. A cross-sectional study to identify a set of risk factors for caprine herpesvirus 1 infection[J]. BMC Vet Res, 2018, 14:94.
[20] TEMPESTA M, PRATELLI A, CORRENTE M, et al. A preliminary study on the pathogenicity of a strain of caprine herpesvirus-1[J]. Comp Immunol Microbiol Infect Dis, 1999, 22(2):137-143.

山羊疱疹病毒Ⅰ型的分离鉴定与致病性

Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain

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